plasmid constructs Search Results


nucleospin 96 flash  (MACHEREY NAGEL)
93
MACHEREY NAGEL nucleospin 96 flash
Nucleospin 96 Flash, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+constructs/pmc04383378-157-5-8?v=MACHEREY+NAGEL
Average 93 stars, based on 1 article reviews
nucleospin 96 flash - by Bioz Stars, 2026-08
93/100 stars
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nucleobond bac 100 kit  (MACHEREY NAGEL)
94
MACHEREY NAGEL nucleobond bac 100 kit
Nucleobond Bac 100 Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+constructs/pmc03433578-90-8-12?v=MACHEREY+NAGEL
Average 94 stars, based on 1 article reviews
nucleobond bac 100 kit - by Bioz Stars, 2026-08
94/100 stars
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thy1 promoter construct  (Addgene inc)
93
Addgene inc thy1 promoter construct
Thy1 Promoter Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+constructs/us12018050-214-1-8?v=Addgene+inc
Average 93 stars, based on 1 article reviews
thy1 promoter construct - by Bioz Stars, 2026-08
93/100 stars
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succinate receptor 1 plasmid construct  (Addgene inc)
93
Addgene inc succinate receptor 1 plasmid construct
Succinate Receptor 1 Plasmid Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+constructs/pmc12796476-296-1-13?v=Addgene+inc
Average 93 stars, based on 1 article reviews
succinate receptor 1 plasmid construct - by Bioz Stars, 2026-08
93/100 stars
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mkate2  (Addgene inc)
93
Addgene inc mkate2
A. Schematic linear map of the AAV-SOX10-OLIG2-gRNA and <t>AAV-mKate2</t> plasmid constructs. B. Schematic of the experimental overview. Animals received either AAV-SOX10-OLIG2-gRNA or AAV-mKate2 via tail vein injection, followed by doxycycline administration in drinking water from D12 to D16. At D14 post-injection, a focal lysolecithin-induced demyelinating lesion was created in the spinal cord. Remyelination was assessed at D40, three weeks after demyelination. C. Representative immunohistochemistry images of lesioned spinal cords at 21 dpl showing SOX10 + PRX + HA - cells (endogenously arising SCs) and SOX10 + PRX + HA + cells (OPC-derived SCs infected by the AAV) in both treatment groups. D. Quantification of HA+ and SOX10 + MPZ + HA - cells in the spinal cord lesion. E. Quantification of SOX10 + MPZ + HA + cells in the spinal cord lesion. F. Immunohistochemistry of the lesion area PRX, MPZ, and GFAP. G. Quantification of (E) shows a significant increase in the number of PRX + , MPZ + , and Sox10 + cells in animals treated with AAV-SOX10-OLIG2-gRNA compared to AAV-mKate2-treated animals. Values are presented as mean ± SD. (*p< 0.05, ns, not significant, by unpaired t-test). The above data was obtained from four biological replicates per group, with at least three fields of view quantified per replicate. H. Immuno-electron microscopy image showing the ultrastructure of an HA + SCs within the spinal cord. The SC body is pseudocoloured yellow, the associated axon in blue and the SC nucleus in red. The inset highlights immunogold particle labelling of HA in the SC nucleus.
Mkate2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+constructs/bio_rxiv__2025__11__24__690179-320-32-35?v=Addgene+inc
Average 93 stars, based on 1 article reviews
mkate2 - by Bioz Stars, 2026-08
93/100 stars
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aav plasmid  (PackGene Biotech lnc)
93
PackGene Biotech lnc aav plasmid
A. Schematic linear map of the AAV-SOX10-OLIG2-gRNA and <t>AAV-mKate2</t> plasmid constructs. B. Schematic of the experimental overview. Animals received either AAV-SOX10-OLIG2-gRNA or AAV-mKate2 via tail vein injection, followed by doxycycline administration in drinking water from D12 to D16. At D14 post-injection, a focal lysolecithin-induced demyelinating lesion was created in the spinal cord. Remyelination was assessed at D40, three weeks after demyelination. C. Representative immunohistochemistry images of lesioned spinal cords at 21 dpl showing SOX10 + PRX + HA - cells (endogenously arising SCs) and SOX10 + PRX + HA + cells (OPC-derived SCs infected by the AAV) in both treatment groups. D. Quantification of HA+ and SOX10 + MPZ + HA - cells in the spinal cord lesion. E. Quantification of SOX10 + MPZ + HA + cells in the spinal cord lesion. F. Immunohistochemistry of the lesion area PRX, MPZ, and GFAP. G. Quantification of (E) shows a significant increase in the number of PRX + , MPZ + , and Sox10 + cells in animals treated with AAV-SOX10-OLIG2-gRNA compared to AAV-mKate2-treated animals. Values are presented as mean ± SD. (*p< 0.05, ns, not significant, by unpaired t-test). The above data was obtained from four biological replicates per group, with at least three fields of view quantified per replicate. H. Immuno-electron microscopy image showing the ultrastructure of an HA + SCs within the spinal cord. The SC body is pseudocoloured yellow, the associated axon in blue and the SC nucleus in red. The inset highlights immunogold particle labelling of HA in the SC nucleus.
Aav Plasmid, supplied by PackGene Biotech lnc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+constructs/pmc11100560-212-7-18?v=PackGene+Biotech+lnc
Average 93 stars, based on 1 article reviews
aav plasmid - by Bioz Stars, 2026-08
93/100 stars
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pen244 ctcf aid 71 114 egfp frt blast frt  (Addgene inc)
93
Addgene inc pen244 ctcf aid 71 114 egfp frt blast frt
A. Schematic linear map of the AAV-SOX10-OLIG2-gRNA and <t>AAV-mKate2</t> plasmid constructs. B. Schematic of the experimental overview. Animals received either AAV-SOX10-OLIG2-gRNA or AAV-mKate2 via tail vein injection, followed by doxycycline administration in drinking water from D12 to D16. At D14 post-injection, a focal lysolecithin-induced demyelinating lesion was created in the spinal cord. Remyelination was assessed at D40, three weeks after demyelination. C. Representative immunohistochemistry images of lesioned spinal cords at 21 dpl showing SOX10 + PRX + HA - cells (endogenously arising SCs) and SOX10 + PRX + HA + cells (OPC-derived SCs infected by the AAV) in both treatment groups. D. Quantification of HA+ and SOX10 + MPZ + HA - cells in the spinal cord lesion. E. Quantification of SOX10 + MPZ + HA + cells in the spinal cord lesion. F. Immunohistochemistry of the lesion area PRX, MPZ, and GFAP. G. Quantification of (E) shows a significant increase in the number of PRX + , MPZ + , and Sox10 + cells in animals treated with AAV-SOX10-OLIG2-gRNA compared to AAV-mKate2-treated animals. Values are presented as mean ± SD. (*p< 0.05, ns, not significant, by unpaired t-test). The above data was obtained from four biological replicates per group, with at least three fields of view quantified per replicate. H. Immuno-electron microscopy image showing the ultrastructure of an HA + SCs within the spinal cord. The SC body is pseudocoloured yellow, the associated axon in blue and the SC nucleus in red. The inset highlights immunogold particle labelling of HA in the SC nucleus.
Pen244 Ctcf Aid 71 114 Egfp Frt Blast Frt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+constructs/pm36482254-312-88-96?v=Addgene+inc
Average 93 stars, based on 1 article reviews
pen244 ctcf aid 71 114 egfp frt blast frt - by Bioz Stars, 2026-08
93/100 stars
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nucleoband xtra bac large contract dna purification kit  (MACHEREY NAGEL)
93
MACHEREY NAGEL nucleoband xtra bac large contract dna purification kit
A. Schematic linear map of the AAV-SOX10-OLIG2-gRNA and <t>AAV-mKate2</t> plasmid constructs. B. Schematic of the experimental overview. Animals received either AAV-SOX10-OLIG2-gRNA or AAV-mKate2 via tail vein injection, followed by doxycycline administration in drinking water from D12 to D16. At D14 post-injection, a focal lysolecithin-induced demyelinating lesion was created in the spinal cord. Remyelination was assessed at D40, three weeks after demyelination. C. Representative immunohistochemistry images of lesioned spinal cords at 21 dpl showing SOX10 + PRX + HA - cells (endogenously arising SCs) and SOX10 + PRX + HA + cells (OPC-derived SCs infected by the AAV) in both treatment groups. D. Quantification of HA+ and SOX10 + MPZ + HA - cells in the spinal cord lesion. E. Quantification of SOX10 + MPZ + HA + cells in the spinal cord lesion. F. Immunohistochemistry of the lesion area PRX, MPZ, and GFAP. G. Quantification of (E) shows a significant increase in the number of PRX + , MPZ + , and Sox10 + cells in animals treated with AAV-SOX10-OLIG2-gRNA compared to AAV-mKate2-treated animals. Values are presented as mean ± SD. (*p< 0.05, ns, not significant, by unpaired t-test). The above data was obtained from four biological replicates per group, with at least three fields of view quantified per replicate. H. Immuno-electron microscopy image showing the ultrastructure of an HA + SCs within the spinal cord. The SC body is pseudocoloured yellow, the associated axon in blue and the SC nucleus in red. The inset highlights immunogold particle labelling of HA in the SC nucleus.
Nucleoband Xtra Bac Large Contract Dna Purification Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+constructs/bio_rxiv__2025__09__19__677276-206-4-12?v=MACHEREY+NAGEL
Average 93 stars, based on 1 article reviews
nucleoband xtra bac large contract dna purification kit - by Bioz Stars, 2026-08
93/100 stars
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nanog luciferase constructs  (Addgene inc)
90
Addgene inc nanog luciferase constructs
A. Schematic linear map of the AAV-SOX10-OLIG2-gRNA and <t>AAV-mKate2</t> plasmid constructs. B. Schematic of the experimental overview. Animals received either AAV-SOX10-OLIG2-gRNA or AAV-mKate2 via tail vein injection, followed by doxycycline administration in drinking water from D12 to D16. At D14 post-injection, a focal lysolecithin-induced demyelinating lesion was created in the spinal cord. Remyelination was assessed at D40, three weeks after demyelination. C. Representative immunohistochemistry images of lesioned spinal cords at 21 dpl showing SOX10 + PRX + HA - cells (endogenously arising SCs) and SOX10 + PRX + HA + cells (OPC-derived SCs infected by the AAV) in both treatment groups. D. Quantification of HA+ and SOX10 + MPZ + HA - cells in the spinal cord lesion. E. Quantification of SOX10 + MPZ + HA + cells in the spinal cord lesion. F. Immunohistochemistry of the lesion area PRX, MPZ, and GFAP. G. Quantification of (E) shows a significant increase in the number of PRX + , MPZ + , and Sox10 + cells in animals treated with AAV-SOX10-OLIG2-gRNA compared to AAV-mKate2-treated animals. Values are presented as mean ± SD. (*p< 0.05, ns, not significant, by unpaired t-test). The above data was obtained from four biological replicates per group, with at least three fields of view quantified per replicate. H. Immuno-electron microscopy image showing the ultrastructure of an HA + SCs within the spinal cord. The SC body is pseudocoloured yellow, the associated axon in blue and the SC nucleus in red. The inset highlights immunogold particle labelling of HA in the SC nucleus.
Nanog Luciferase Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+constructs/pmc03916758__mmc1-141-2-8?v=Addgene+inc
Average 90 stars, based on 1 article reviews
nanog luciferase constructs - by Bioz Stars, 2026-08
90/100 stars
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p1 eyfp pa  (Addgene inc)
90
Addgene inc p1 eyfp pa
A. Schematic linear map of the AAV-SOX10-OLIG2-gRNA and <t>AAV-mKate2</t> plasmid constructs. B. Schematic of the experimental overview. Animals received either AAV-SOX10-OLIG2-gRNA or AAV-mKate2 via tail vein injection, followed by doxycycline administration in drinking water from D12 to D16. At D14 post-injection, a focal lysolecithin-induced demyelinating lesion was created in the spinal cord. Remyelination was assessed at D40, three weeks after demyelination. C. Representative immunohistochemistry images of lesioned spinal cords at 21 dpl showing SOX10 + PRX + HA - cells (endogenously arising SCs) and SOX10 + PRX + HA + cells (OPC-derived SCs infected by the AAV) in both treatment groups. D. Quantification of HA+ and SOX10 + MPZ + HA - cells in the spinal cord lesion. E. Quantification of SOX10 + MPZ + HA + cells in the spinal cord lesion. F. Immunohistochemistry of the lesion area PRX, MPZ, and GFAP. G. Quantification of (E) shows a significant increase in the number of PRX + , MPZ + , and Sox10 + cells in animals treated with AAV-SOX10-OLIG2-gRNA compared to AAV-mKate2-treated animals. Values are presented as mean ± SD. (*p< 0.05, ns, not significant, by unpaired t-test). The above data was obtained from four biological replicates per group, with at least three fields of view quantified per replicate. H. Immuno-electron microscopy image showing the ultrastructure of an HA + SCs within the spinal cord. The SC body is pseudocoloured yellow, the associated axon in blue and the SC nucleus in red. The inset highlights immunogold particle labelling of HA in the SC nucleus.
P1 Eyfp Pa, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+constructs/pm33930275__sb1c00037_si_001-143-41-42?v=Addgene+inc
Average 90 stars, based on 1 article reviews
p1 eyfp pa - by Bioz Stars, 2026-08
90/100 stars
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nucleobond xtra bac columns  (MACHEREY NAGEL)
92
MACHEREY NAGEL nucleobond xtra bac columns
A. Schematic linear map of the AAV-SOX10-OLIG2-gRNA and <t>AAV-mKate2</t> plasmid constructs. B. Schematic of the experimental overview. Animals received either AAV-SOX10-OLIG2-gRNA or AAV-mKate2 via tail vein injection, followed by doxycycline administration in drinking water from D12 to D16. At D14 post-injection, a focal lysolecithin-induced demyelinating lesion was created in the spinal cord. Remyelination was assessed at D40, three weeks after demyelination. C. Representative immunohistochemistry images of lesioned spinal cords at 21 dpl showing SOX10 + PRX + HA - cells (endogenously arising SCs) and SOX10 + PRX + HA + cells (OPC-derived SCs infected by the AAV) in both treatment groups. D. Quantification of HA+ and SOX10 + MPZ + HA - cells in the spinal cord lesion. E. Quantification of SOX10 + MPZ + HA + cells in the spinal cord lesion. F. Immunohistochemistry of the lesion area PRX, MPZ, and GFAP. G. Quantification of (E) shows a significant increase in the number of PRX + , MPZ + , and Sox10 + cells in animals treated with AAV-SOX10-OLIG2-gRNA compared to AAV-mKate2-treated animals. Values are presented as mean ± SD. (*p< 0.05, ns, not significant, by unpaired t-test). The above data was obtained from four biological replicates per group, with at least three fields of view quantified per replicate. H. Immuno-electron microscopy image showing the ultrastructure of an HA + SCs within the spinal cord. The SC body is pseudocoloured yellow, the associated axon in blue and the SC nucleus in red. The inset highlights immunogold particle labelling of HA in the SC nucleus.
Nucleobond Xtra Bac Columns, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+constructs/pmc08266218-116-9-8?v=MACHEREY+NAGEL
Average 92 stars, based on 1 article reviews
nucleobond xtra bac columns - by Bioz Stars, 2026-08
92/100 stars
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j hanna 30  (Addgene inc)
91
Addgene inc j hanna 30
A. Schematic linear map of the AAV-SOX10-OLIG2-gRNA and <t>AAV-mKate2</t> plasmid constructs. B. Schematic of the experimental overview. Animals received either AAV-SOX10-OLIG2-gRNA or AAV-mKate2 via tail vein injection, followed by doxycycline administration in drinking water from D12 to D16. At D14 post-injection, a focal lysolecithin-induced demyelinating lesion was created in the spinal cord. Remyelination was assessed at D40, three weeks after demyelination. C. Representative immunohistochemistry images of lesioned spinal cords at 21 dpl showing SOX10 + PRX + HA - cells (endogenously arising SCs) and SOX10 + PRX + HA + cells (OPC-derived SCs infected by the AAV) in both treatment groups. D. Quantification of HA+ and SOX10 + MPZ + HA - cells in the spinal cord lesion. E. Quantification of SOX10 + MPZ + HA + cells in the spinal cord lesion. F. Immunohistochemistry of the lesion area PRX, MPZ, and GFAP. G. Quantification of (E) shows a significant increase in the number of PRX + , MPZ + , and Sox10 + cells in animals treated with AAV-SOX10-OLIG2-gRNA compared to AAV-mKate2-treated animals. Values are presented as mean ± SD. (*p< 0.05, ns, not significant, by unpaired t-test). The above data was obtained from four biological replicates per group, with at least three fields of view quantified per replicate. H. Immuno-electron microscopy image showing the ultrastructure of an HA + SCs within the spinal cord. The SC body is pseudocoloured yellow, the associated axon in blue and the SC nucleus in red. The inset highlights immunogold particle labelling of HA in the SC nucleus.
J Hanna 30, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+constructs/pm37611093-466-7-10?v=Addgene+inc
Average 91 stars, based on 1 article reviews
j hanna 30 - by Bioz Stars, 2026-08
91/100 stars
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Image Search Results


A. Schematic linear map of the AAV-SOX10-OLIG2-gRNA and AAV-mKate2 plasmid constructs. B. Schematic of the experimental overview. Animals received either AAV-SOX10-OLIG2-gRNA or AAV-mKate2 via tail vein injection, followed by doxycycline administration in drinking water from D12 to D16. At D14 post-injection, a focal lysolecithin-induced demyelinating lesion was created in the spinal cord. Remyelination was assessed at D40, three weeks after demyelination. C. Representative immunohistochemistry images of lesioned spinal cords at 21 dpl showing SOX10 + PRX + HA - cells (endogenously arising SCs) and SOX10 + PRX + HA + cells (OPC-derived SCs infected by the AAV) in both treatment groups. D. Quantification of HA+ and SOX10 + MPZ + HA - cells in the spinal cord lesion. E. Quantification of SOX10 + MPZ + HA + cells in the spinal cord lesion. F. Immunohistochemistry of the lesion area PRX, MPZ, and GFAP. G. Quantification of (E) shows a significant increase in the number of PRX + , MPZ + , and Sox10 + cells in animals treated with AAV-SOX10-OLIG2-gRNA compared to AAV-mKate2-treated animals. Values are presented as mean ± SD. (*p< 0.05, ns, not significant, by unpaired t-test). The above data was obtained from four biological replicates per group, with at least three fields of view quantified per replicate. H. Immuno-electron microscopy image showing the ultrastructure of an HA + SCs within the spinal cord. The SC body is pseudocoloured yellow, the associated axon in blue and the SC nucleus in red. The inset highlights immunogold particle labelling of HA in the SC nucleus.

Journal: bioRxiv

Article Title: Spontaneous differentiation across cell lineages of separate germ layer origin during progenitor cell-mediated regeneration of the central nervous system

doi: 10.1101/2025.11.24.690179

Figure Lengend Snippet: A. Schematic linear map of the AAV-SOX10-OLIG2-gRNA and AAV-mKate2 plasmid constructs. B. Schematic of the experimental overview. Animals received either AAV-SOX10-OLIG2-gRNA or AAV-mKate2 via tail vein injection, followed by doxycycline administration in drinking water from D12 to D16. At D14 post-injection, a focal lysolecithin-induced demyelinating lesion was created in the spinal cord. Remyelination was assessed at D40, three weeks after demyelination. C. Representative immunohistochemistry images of lesioned spinal cords at 21 dpl showing SOX10 + PRX + HA - cells (endogenously arising SCs) and SOX10 + PRX + HA + cells (OPC-derived SCs infected by the AAV) in both treatment groups. D. Quantification of HA+ and SOX10 + MPZ + HA - cells in the spinal cord lesion. E. Quantification of SOX10 + MPZ + HA + cells in the spinal cord lesion. F. Immunohistochemistry of the lesion area PRX, MPZ, and GFAP. G. Quantification of (E) shows a significant increase in the number of PRX + , MPZ + , and Sox10 + cells in animals treated with AAV-SOX10-OLIG2-gRNA compared to AAV-mKate2-treated animals. Values are presented as mean ± SD. (*p< 0.05, ns, not significant, by unpaired t-test). The above data was obtained from four biological replicates per group, with at least three fields of view quantified per replicate. H. Immuno-electron microscopy image showing the ultrastructure of an HA + SCs within the spinal cord. The SC body is pseudocoloured yellow, the associated axon in blue and the SC nucleus in red. The inset highlights immunogold particle labelling of HA in the SC nucleus.

Article Snippet: A second construct was generated using the same AAV backbone (AAV-CMVc-Cas9, Addgene #106431) and Sox10 U2 promoter, combined with rtTA, a T2A self-cleaving peptide, and WPRE elements (from pHR-EF1α-Tet-on 3G), together with mKate2 (from NFKBRp-mKate2-2xNLS-p2a-puroR, Addgene #82024) to enable fluorescent reporting of promoter activity.

Techniques: Plasmid Preparation, Construct, Injection, Immunohistochemistry, Derivative Assay, Infection, Immuno-Electron Microscopy

A. Immunostaining of OLIG2, IBA1 and MBP in the lesion area. B. Quantification of the data presented in A. The bar graphs show the lesion size and the number of OLIG2 + , MBP + , and IBA1 + cells in animals receiving AAV-SOX10-OLIG2-gRNA or AAV-mKate2-treated animals. Values are expressed as mean ± SD. Statistical significance was determined by one-way ANOVA with Tukey’s multiple comparisons test. C. Quantification of the spatial relationship between PRX + SCs and GFAP + astrocytes in the lesion site.

Journal: bioRxiv

Article Title: Spontaneous differentiation across cell lineages of separate germ layer origin during progenitor cell-mediated regeneration of the central nervous system

doi: 10.1101/2025.11.24.690179

Figure Lengend Snippet: A. Immunostaining of OLIG2, IBA1 and MBP in the lesion area. B. Quantification of the data presented in A. The bar graphs show the lesion size and the number of OLIG2 + , MBP + , and IBA1 + cells in animals receiving AAV-SOX10-OLIG2-gRNA or AAV-mKate2-treated animals. Values are expressed as mean ± SD. Statistical significance was determined by one-way ANOVA with Tukey’s multiple comparisons test. C. Quantification of the spatial relationship between PRX + SCs and GFAP + astrocytes in the lesion site.

Article Snippet: A second construct was generated using the same AAV backbone (AAV-CMVc-Cas9, Addgene #106431) and Sox10 U2 promoter, combined with rtTA, a T2A self-cleaving peptide, and WPRE elements (from pHR-EF1α-Tet-on 3G), together with mKate2 (from NFKBRp-mKate2-2xNLS-p2a-puroR, Addgene #82024) to enable fluorescent reporting of promoter activity.

Techniques: Immunostaining